DcR2 Rabbit Polyclonal Antibody

• Catalog Number: orb10524
• Category: Antibodies
• Description: DcR2 Rabbit Polyclonal Antibody
• Target: TNFRSF10D
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human DcR2 (56-160/386aa)
• UniProt ID: Q9UBN6
• RRID: AB_10751573
• MW: 36 kDa
• Tested applications: FC, WB
• Dilution range: WB=1:500-2000, Flow-Cyt=1μg /test
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: TNFRSF10D; Decoy receptor 2; CD 264; CD264; CD-264
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: Jurkat cells (blue). Primary Antibody: Rabbit Anti-DcR2 antibody (orb10524), Dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol, The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (orb10524, 1 µg/1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20000 events was performed.

Sample: Lane 1: Human MKN45 cell lysates, Lane 2: Human SW480 cell lysates, Lane 3: Human HUVEC cell lysates, Lane 4: Human DU145 cell lysates, Lane 5: Human HepG2 cell lysates, Primary: Anti-DcR2 (orb10524) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 36 kDa, Observed band size: 50 kDa.