DC-SIGN Antibody

• Catalog Number: orb248128
• Category: Antibodies
• Description: DC-SIGN (Dendritic Cell-Specific Intercellular adhesion molecule-3-Grabbing Non-integrin) also known as CD209, is part of the Lectin family of proteins. DC-SIGN is part of the innate immune response via its modulation of Toll-like Receptors. It is present on the surface of macrophages and dendritic cells and binds mannose carbohydrates, a class of PAMPS found on bacteria, fungi and viruses. PAMP stimulated DC-SIGN activates the Raf kinases Raf-1 and B-Raf. Raf-1 phosphorylates Ser276 of NF-kB p65 which serves as a binding site for histone acetyltransferases CREB-binding protein (CBP) and p300. Acetylation of NF-kB p65 leads to enhanced and prolonged anti-inflammatory cytokine IL-10 production.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Immunogen: A recombinant protein fragment from human DC-SIGN was used as the immunogen for this antibody.
• Dilution range: Western blot: 2-5ug/ml
• Purity: Protein A purified antibody
• Conjugation: Unconjugated
• Formula: 0.5 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This DC-SIGN antibody is available for research use only.
• Entrez: 30835
• Storage: Aliquot the DC-SIGN antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: 1X PBS, pH 7.4
• Note: For research use only
• Application notes: Provided assay concentrations are suggestions only, DC-SIGN antibody titration may be required for optimal results.
• Expiration Date: 12 months from date of receipt.

Western blot testing of human samples with DC-SIGN antibody at 2 ug/ml. Predicted molecular weight ~46 kDa.