DC-SIGN Antibody / CD209

• Catalog Number: orb2640310
• Category: Antibodies
• Description: DC-SIGN / CD209 is a transmembrane receptor that is expressed on the surface of dendritic cells and macrophages. It is involved in the innate immune system and recognizes numerous evolutionarily divergent pathogens ranging from parasites to viruses. The protein is organized into three distinct domains: an N-terminal transmembrane domain, a tandem-repeat neck domain and C-type lectin carbohydrate recognition domain. The extracellular region consisting of the C-type lectin and neck domains has a dual function as a pathogen recognition receptor and a cell adhesion receptor by binding carbohydrate ligands on the surface of microbes and endogenous cells. The neck region is important for homo-oligomerization, which allows the receptor to bind multivalent ligands with high avidity.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: C209/1781
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG2b, kappa
• Immunogen: A human partial recombinant protein was used as the immunogen for the DC-SIGN antibody.
• Antibody Type: Primary Antibody
• Dilution range: Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This DC-SIGN antibody is available for research use only.
• UniProt ID: Q9NNX6
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Titering of the DC-SIGN antibody may be required for optimal performance.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human lung carcinoma with DC-SIGN antibody (clone C209/1781). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH9, for 10-20.

IHC testing of FFPE human small intestine with DC-SIGN antibody (clone C209/1781). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH9, for 10-20.

IHC testing of FFPE human colon carcinoma with DC-SIGN antibody (clone C209/1781). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH9, for 10-20.

SDS-PAGE Analysis of Purified, BSA-Free DC-SIGN Antibody (clone C209/1781). Confirmation of Integrity and Purity of the Antibody.

Western blot testing of human liver and spleen lysate with DC-SIGN antibody. Predicted molecular weight ~46 kDa.