DBH Antibody / Dopamine beta Hydroxylase

• Catalog Number: orb1822680
• Category: Antibodies
• Description: Dopamine beta-hydroxylase (DBH) catalyzes the conversion of dopamine to noradrenaline in the biosynthesis of catecholamines. DBH is selectively expressed in noradrenergic and adrenergic neurons, as well as in neuroendocrine cells, and it serves as a specific protein marker for noradrenergic processes. The active form of DBH is a homotetramer, which is found in the lumen of synaptic vesicles of corresponding nerve cells, where it localizes to both the membrane and cytosol. DBH is induced by nerve growth factor and Insulin growth factor-1 and is regulated by intracellular second messengers protein kinase A, cyclic AMP, diacyl glycerol and Ca2+. Expression of DBH is transcriptionally mediated by Sp1, CREB and AP-1 proteins including c-Fos, c-Jun and JunD.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG2, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: A recombinant partial protein sequence (within amino acids 200-400) from the human protein was used as the immunogen for the DBH antibody.
• UniProt ID: P09172
• Tested applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Antibody Type: Primary Antibody
• Clone Number: DBH/7224
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This DBH antibody is available for research use only.
• Note: For research use only

IHC staining of FFPE human adrenal gland tissue with Dopamine Beta-Hydroxylase antibody (clone DBH/7224). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.