DAXX Antibody

SKU: orb345563

Description

DAXX antibody

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:7,000 – 1:45,000, WB: 1:200 – 1:2000
• Reactivity: Human
• Application Notes:
  This affinity-purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 81 kDa in size corresponding to DAXX protein by western blotting in the appropriate cell lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 250-275 of human DAXX protein.
• Target: DAXX
• Purity: This affinity-purified antibody is directed against human DAXX protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross reactivity with DAXX protein from human, mouse, and rat based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.06 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-DAXX Antibody, BING2 antibody, BING-2, DAP 6 antibody, DAP6 antibody, Death associated protein 6 antibody, Death domain associated protein 6 antibody, Fas death domain-associated protein, ETS1-associated protein 1, EAP1

Western blot using Biorbyt's affinity purified anti-DAXX antibody shows detection of DAXX in RWPE-1 cell extracts. The membrane was probed with the primary antibody diluted to 1:200, and further probed with peroxidase conjugated anti-Rabbit IgG at a 1:25000 dilution.

Quick Database Links

Gene Symbol

DAXX

UniProt

Q9UER7

RefSeq:4503257