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Catalog Number | orb1256166 |
---|---|
Category | Antibodies |
Description | DARS2 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, WB |
Reactivity | Human, Mouse |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 406-645 of human DARS2 (NP_060592.2). |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IHC: 1:50 - 1:200 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 74kDa |
Target | DARS2 |
UniProt ID | Q6PI48 |
Storage | Store at -20°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
Alternative names | ASPRS, LBSL, MT-ASPRS, mtAspRS, aspartate--tRNA li Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using DARS2 antibody (orb1256166) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human liver damage using DARS2 antibody (orb1256166) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using DARS2 antibody (orb1256166) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using DARS2 antibody (orb1256166) at dilution of 1:100 (40x lens).
ELISA, FC, ICC, IF, IHC, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
WB | |
Bovine, Mouse | |
Human, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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