DARS Antibody (N-term)

SKU: orb29039

Description

Purified rabbit polyclonal antibody.

Research Area

Epigenetics

Images & Validation

• Tested Applications: FC, IF, IHC-P, WB
• Dilution Range: WB: 1:1000, IHC-P: 1:50-100, IF/ICC: 1:10-50, FACS: 1:10-50
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB14801
• Immunogen: Synthetic Peptide
• Target: DARS1
• Molecular Weight: 57136
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-Aspartyl-tRNA synthetase antibody, anti-cytoplasmic, DARS, antibody

Confocal immunofluorescent analysis of DARS Antibody (N-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

DARS Antibody (N-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

DARS Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of DARS Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

DARS Antibody (N-term) western blot analysis in Jurkat cell line lysates (35 ug/lane). This demonstrates the DARS antibody detected the DARS protein (arrow).

Western blot analysis of DARS (arrow) using rabbit polyclonal DARS Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DARS gene.

Quick Database Links

Gene Symbol

DARS1

UniProt

P14868

RefSeq (Protein):NP_001340.2