DARC Rabbit Polyclonal Antibody

SKU: orb221394

Description

DARC Rabbit Polyclonal Antibody

Research Area

CC Chemokines, CXC Chemokines, Cardiovascular Research, G Protein-Coupled Receptors, GPCR Signaling Pathway, Immunology, Innate Immunity

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Rat
• Predicted Reactivity: Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human DARC (61-150/336aa)
• Target: ACKR1
• Molecular Weight: 37 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

CCBP1; CD234; CD234 antigen; DARC; Dfy; Duffy antigen/chemokine receptor; Duffy blood group; DUFFY_MOUSE; FY antibody Fy glycoprotein; Glycoprotein D; GPD; GpFy; Human Duffy blood group antigen(Fya-b+) mRNA complete cds; Plasmodium vivax receptor; WBCQ1; DUFFY_MOUSE.

Tissue/Cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-DARC Polyclonal Antibody, Unconjugated (orb221394) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: Rat colon tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-DARC Polyclonal Antibody, Unconjugated (orb221394) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

ACKR1

UniProt

Q16570