DARC Antibody

• Catalog Number: orb1247588
• Category: Antibodies
• Description: DARC Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IHC, WB
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: HRAELSPSTENSSQLDFED-C
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 37kDa band observed in Human Liver lysates (calculated MW of 35.6kDa according to NP_002027.2). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:In paraffin embedded Human Kidney stains epithelial cells of renal tubules and endothelial cells of vessels. The granular staining is compatible with the endoplasmic reticulum association of the protein. Recommended concentration: 3ug/ml. Paraffin embedded Human Lung. Recommended concentration: 5ug/ml.Flow cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml. A customer has reported successful staining of HEK293S-TetR transfected with DARC receptor at a dilution of 1/1000.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: DARC
• UniProt ID: Q16570
• NCBI: NP_001116423.1, NP_002027.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: FY, DARC, HGNC:4035, Duffy blood group, CCBP1, GPD
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 37kDa band observed in Human Liver lysates (calculated MW of 35.6kDa according to NP_002027.2). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:In paraffin embedded Human Kidney stains epithelial cells of renal tubules and endothelial cells of vessels. The granular staining is compatible with the endoplasmic reticulum association of the protein. Recommended concentration: 3ug/ml. Paraffin embedded Human Lung. Recommended concentration: 5ug/ml.Flow cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml. A customer has reported successful staining of HEK293S-TetR transfected with DARC receptor at a dilution of 1/1000.
• Expiration Date: 12 months from date of receipt.

orb1247588 (0.3 ug/ml) staining of Human Liver lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247588 (3 ug/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with citrate buffer Ph 6, HRP-staining.

orb1247588 (5 ug/ml) staining of paraffin embedded Human Lung. Microwaved antigen retrieval with citrate buffer Ph 6, AP-staining.

Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (0.4 ug/ml). IgG control: Unimmunized goat IgG (black line).