DARC Antibody [2C3], Rabbit IgG

• Catalog Number: orb1671828
• Category: Antibodies
• Description: DARC Antibody [2C3], Rabbit IgG
• Clonality: Recombinant
• Clone Number: 2C3
• Tested applications: Blocking, ELISA, FC
• Reactivity: Human
• Isotype: IgG kappa
• Immunogen: CHO cells transfected with cDNA encoding the Duffy glycoprotein.
• Concentration: batch dependent
• Conjugation: Unconjugated
• UniProt ID: Q4VBN9
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: Fy6, Duffy Antigen Receptor for Chemokines, NaM185
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot using anti-DARC antibody 2C3. Human liver lysate samples (35 ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rsion of 2C3 (orb1671828) at 0.03 ug/ml before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for DARC is 35.6kDa. orb1671828 successfully detected DARC in human liver lysate.

Immunofluorescence staining of fixed HepG2 cells with anti-DARC antibody 2C3. Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells on Shi-fix™ coverslips- permewith 0.15% Triton and stained with the chimeric r version of 2C3 (orb1671828) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 ug/ml)- showing cytoplasmic staining- likely of endosomes- and some membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671828- DAPI- merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody (0) followed by Alexa Fluor® 488 secondary antibody.