DAP3 antibody

• Catalog Number: orb18383
• Category: Antibodies
• Description: Goat polyclonal antibody to DAP3
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC, WB
• Reactivity: Human
• Dilution range: ELISA: 1:64000, WB: 0.1-1 μg/ml, IHC-P: 10 μg/ml
• Conjugation: Unconjugated
• MW: 45.6; 41.7; 41.0
• Target: DAP3
• Entrez: 7818
• Protein Sequence: NPSLLERHCAYL
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti DAP3 antibody, anti death associated protein
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 40kDa band observed in lysates of cell line HeLa (calculated MW of 45.6kDa according to NP_387506.1). In transfected HEK293 transiently expressing DAP3 a band of approx. 48kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3 μg/ml.
• Expiration Date: 12 months from date of receipt.

Immunohistochemical staining of Human Tonsil using DAP3 antibody

Immunohistochemical staining of HeLa lysate using DAP3 antibody

Western blot analysis of HeLa lysate using DAP3 antibody

Western blot analysis of HEK293 lysate using DAP3 antibody

Immunofluorescence analysis of guanidinium thiocyanate lysate using DAP3 antibody

Immunofluorescence analysis of MCF7 cells using DAP3 antibody

Western blot analysis of HeLa lysate using DAP3 antibody