DAL1/EPB41L3 Rabbit Polyclonal Antibody

SKU: orb100700

Description

DAL1/EPB41L3 Rabbit Polyclonal Antibody

Research Area

Actin Binding Proteins, Cancer Research, Cell Biology, Tumor Suppressors

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Porcine, Rabbit, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human DAL1/EPB41L3 (131-230/1087aa)
• Target: EPB41L3
• Molecular Weight: 120 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Differentially expressed in adenocarcinoma of the lung protein 1; 4.1B; Band 4.1 like protein 3; DAL 1; DAL1; EPB41L3; Erythrocyte membrane protein band 4.1 like 3; HGNC:3380; KIAA0987; E41L3_HUMAN.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (DAL1 (EPB41L3)) Polyclonal Antibody, Unconjugated (orb100700) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (DAL1 (EPB41L3)) Polyclonal Antibody, Unconjugated (orb100700) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Human HUVEC cell lysates, Lane 2: Human U-2 OS cell lysates, Primary: Anti-DAL1/EPB41L3 (orb100700) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 120 kDa, Observed band size: 120 kDa.

Quick Database Links

Gene Symbol

EPB41L3

UniProt

Q9Y2J2