DACT1 Rabbit Polyclonal Antibody

• Catalog Number: orb182970
• Category: Antibodies
• Description: DACT1 Rabbit Polyclonal Antibody
• Target: DACT1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Equine, Mouse, Rabbit, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: KLH conjugated synthetic peptide derived from human DACT1 (751-836/836aa)
• UniProt ID: Q9NYF0
• MW: 90 kDa
• Tested applications: ICC
• Dilution range: ICC/IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Dact1/Dapper homolog 1; Dact; Dact1; DACT1_HUMAN;
• Note: For research use only

Tissue/Cell: human HepG2 cells, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-DACT1 Polyclonal Antibody, Unconjugated (orb182970) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated (orb868589) used at 1:200 dilution for 40 minutes at 37°C.