Cytokeratin 8/18 Antibody

• Catalog Number: orb750034
• Category: Antibodies
• Description: Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This mAb recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells; there is very little keratin 18 in the normal epidermis so only Paget cells are stained.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 5D3
• Tested applications: FACS, IF, IHC-P
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: A cytoskeleton preparation from human breast cancer MCF-7 cells was used as the immunogen for the Cytokeratin 8/18 antibody.
• Dilution range: Flow cytometry: 2-5ul/million cells,Immunofluorescence: 1:100-1:200,Immunohistochemistry (FFPE): 1:50-1:100 for 30 min at RT
• Purity: Unpurified high titer supernatant
• Conjugation: Other
• Formula: Bioreactor concentrate with 0.05% sodium azide
• Hazard Information: This Cytokeratin 8/18 antibody is available for research use only.
• UniProt ID: P05787, P05783
• Storage: Store the Cytokeratin 8/18 antibody at 2-8°C.
• Buffer/Preservatives: Bioreactor concentrate with 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the Cytokeratin 8/18 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC analysis of FFPE human colon carcinoma with Cytokeratin 8/18 antibody (clone 5D3). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.

IHC analysis of FFPE human prostate carcinoma with Cytokeratin 8/18 antibody (clone 5D3). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.