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Catalog Number | orb43712 |
---|---|
Category | Antibodies |
Description | Mouse Monoclonal to Cytokeratin 8. |
Clonality | Monoclonal |
Clone Number | C-43 |
Tested applications | FC, ICC, IHC-P, IP, WB |
Reactivity | Bovine, Human, Porcine, Rabbit, Sheep |
Isotype | Mouse IgG1 |
Immunogen | Cytoskeleton preparation from HeLa human cervix carcinoma cell line. |
Concentration | 1 mg/ml |
Dilution range | Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: prostate. Flow cytometry: Recommended dilution: 1-5 μg/ml, intracellular staining. |
Purity | Purified by protein-A affinity chromatography. |
Conjugation | Unconjugated |
Target | Cytokeratin 8 |
Entrez | 3856 |
UniProt ID | Q7L4M3 |
Storage | Store at 2-8°C. Do not freeze. |
Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Alternative names | Anti-Cytokeratin 8 antibody Read more... |
Note | For research use only |
Application notes | Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: prostate. Flow cytometry: Recommended dilution: 1-5 μg/ml, intracellular staining. |
Expiration Date | 12 months from date of receipt. |
Western blotting detection of cytokeratin 8 in HeLa cell lysate (reducing conditions) by mouse monoclonal antibody C-43.
Immunocytochemistry staining of cytokeratin 8 in Hep-2 cells using mouse monoclonal C-43 (orb43712, diluted 1:100), detected with GAM IgG-Alexa Fluor®488 (diluted 1:200; green).
Immunohistochemistry staining of human prostate (paraffin sections) using anti-cytokeratin 8(C-43).
FC, ICC, IF, IHC, IHC-Fr, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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