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Cytokeratin 3+12 Rabbit Polyclonal Antibody

SKU: orb4935

Description

Rabbit Anti-Cytokeratin 3+12 antibody

Research Area

Autoimmune, Immunology, Signal Transduction, Visual System

Images & Validation

Tested ApplicationsELISA, IF, IHC-Fr, IHC-P, WB
Dilution RangeWB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
ReactivityHuman
Predicted ReactivityBovine, Canine, Equine, Mouse, Porcine, Rabbit, Rat

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human Cytokeratin 3+12
TargetKRT3
Molecular Weight65+54 kDa
PurificationAffinity purified by Protein A
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

CK3; K3; MECD2; K2C3_HUMAN; KRT3; 65 kDa cytokeratin; Cytokeratin-3 (CK-3); Keratin-3 (K3); Type-II keratin Kb3; K2C3_RABIT;

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Cytokeratin 3+12 Rabbit Polyclonal Antibody

Immunohistochemical analysis of formalin-fixed and paraffin embedded mouse embryo tissue ( dilution at:1:200) using Cytokeratin 3/12 antibody

UniProt Details

No UniProt data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol
IHC-Fr
Immunohistochemistry Frozen
View Protocol
IF
Immunofluorescence
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

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Yang Yu 1, Andrey Yurevich Andreev 1 2 3, Olga Sergeevna Rogovaya 4, Anastasia Mikhailovna Subbot 5, Sergey Petrovich Domogatsky 3 6, Sergey Eduardovich Avetisov 1 2, Ekaterina Andreevna Vorotelyak 4, Egor Olegovich Osidak 3 7 Matrix-Assisted Cell Transplantation for the Treatment of Limbal Stem Cell Deficiency in a Rabbit Model Biomedicines, 12(1), 101 (2024)

Applications

IF

Reactivity

Rabbit

Se-Jeong Kim 1, Ning Guo 1, Zong Yao Tan 1, Xing Gao 1, Pouria Fattahi 1, Haijiao Liu 1, Jeehan Chang 1, Mousa Younesi 1 2, Selice Jung 1, Yewhan Chung 1, Minkyung Song 1, Michelle Jung 1, Dan Dongeun Huh A Bioengineered Model of the Human Cornea for Preclinical Assessment of Human Ocular Exposure to Environmental Toxicants Adv Healthc Mater, (2025)

Cytokeratin 3+12 Rabbit Polyclonal Antibody (orb4935)

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Available Sizes

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50 μl
$ 250.00
100 μl
$ 390.00
200 μl
$ 470.00
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