Cytokeratin 3+12 Rabbit Polyclonal Antibody

SKU: orb4935

Description

Rabbit Anti-Cytokeratin 3+12 antibody

Research Area

Autoimmune, Immunology, Signal Transduction, Visual System

Images & Validation

• Tested Applications: ELISA, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Equine, Mouse, Porcine, Rabbit, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Cytokeratin 3+12
• Target: KRT3
• Molecular Weight: 65+54 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

CK 12; CK 3; CK12; CK3; Cytokeratin 12; Cytokeratin 3; K12; K3; keratin 12(Meesmann corneal dystrophy); Keratin 12; Keratin 3; Keratin, type I cytoskeletal 12; Keratin, type II cytoskeletal 3; KRT12; KRT3; 65 kDa cytokeratin.

Immunohistochemical analysis of formalin-fixed and paraffin embedded mouse embryo tissue ( dilution at:1:200) using Cytokeratin 3/12 antibody

Quick Database Links

Gene Symbol

KRT3

UniProt

P12035

References

Yang Yu 1, Andrey Yurevich Andreev 1 2 3, Olga Sergeevna Rogovaya 4, Anastasia Mikhailovna Subbot 5, Sergey Petrovich Domogatsky 3 6, Sergey Eduardovich Avetisov 1 2, Ekaterina Andreevna Vorotelyak 4, Egor Olegovich Osidak 3 7 Matrix-Assisted Cell Transplantation for the Treatment of Limbal Stem Cell Deficiency in a Rabbit Model Biomedicines, 12(1), 101 (2024)

PubMed: 38255207

Applications

IF

Reactivity

Rabbit

Se-Jeong Kim 1, Ning Guo 1, Zong Yao Tan 1, Xing Gao 1, Pouria Fattahi 1, Haijiao Liu 1, Jeehan Chang 1, Mousa Younesi 1 2, Selice Jung 1, Yewhan Chung 1, Minkyung Song 1, Michelle Jung 1, Dan Dongeun Huh A Bioengineered Model of the Human Cornea for Preclinical Assessment of Human Ocular Exposure to Environmental Toxicants Adv Healthc Mater, (2025)

PubMed: 39838783