Cytokeratin 19 antibody

• Catalog Number: orb43666
• Category: Antibodies
• Description: Mouse Monoclonal to Cytokeratin 19.
• Clonality: Monoclonal
• Clone Number: BA-17
• Tested applications: FC, ICC, IHC-P, IP, WB
• Reactivity: Human
• Isotype: Mouse IgG1 kappa
• Immunogen: Mammary organoids.
• Concentration: 1 mg/ml
• Dilution range: Flow cytometry: Recommended dilution: 1-5 μg/ml. Intracellular staining.Immunohistochemistry: Recommended dilution: 5-10 μg/ml.Western blotting: Recommended dilution: 1-2 μg/ml.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: Cytokeratin 19
• Entrez: 3880
• UniProt ID: P08727
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-Cytokeratin 19 antibody
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 1-5 μg/ml. Intracellular staining.Immunohistochemistry: Recommended dilution: 5-10 μg/ml.Western blotting: Recommended dilution: 1-2 μg/ml.
• Expiration Date: 12 months from date of receipt.

Separation of MCF-7 cells (red-filled) from human leukocytes (black-dashed) in flow cytometry analysis (intracellular staining) of peripheral whole blood spiked with MCF-7 cells stained using anti-Cytokeratin 19 (BA-17) purified antibody (concentration in sample 3 µg/ml, GAM APC).

Immunohistochemistry staining of human colon (paraffin sections) using anti-cytokeratin 19 (BA-17).

Immunohistochemistry staining (paraffin sections) of cytokeratin 19 in human liver using mouse monoclonal antibody BA-17 (orb43666, diluted 1:100), detected with GAM IgG-Alexa Fluor®488 (diluted 1:200; green), cell nuclei stained with PI (1 µg/ml; orange).

Western blotting analysis of human cytokeratin 19 using mouse monoclonal antibody BA-17 on lysates of HT-29 cell line and MOLT-4 cell line (cytokeratin non-expressing cell line; negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-cytokeratin 19 monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. A specific band was detected for cytokeratin 19 at approximately 41 kDa.