Cytokeratin 14 Antibody / CK14 / KRT14

• Catalog Number: orb1151530
• Category: Antibodies
• Description: Mouse monoclonal antibody to Cytokeratin 14
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: KRT14/4132
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Mouse IgG2c, kappa
• Immunogen: A portion of amino acids 351-472 was used as the immunogen for the Cytokeratin 14 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This Cytokeratin 14 antibody is available for research use only.
• UniProt ID: P02533
• Storage: Aliquot the Cytokeratin 14 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the Cytokeratin 14 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human prostate tissue with Cytokeratin 14 antibody (clone KRT14/4132). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human prostate tissue with Cytokeratin 14 antibody (clone KRT14/4132). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free Cytokeratin 14 antibody (clone KRT14/4132) as confirmation of integrity and purity.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using Cytokeratin 14 antibody (clone KRT14/4132). These results demonstrate the foremost specificity of the KRT14/4132 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.