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Cytochrome P450 2B6 Rabbit Polyclonal Antibody

Catalog Number: orb5949

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb5949
CategoryAntibodies
DescriptionCytochrome P450 2B6 Rabbit Polyclonal Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsFC, WB
Predicted ReactivityCanine, Rabbit, Rat
ReactivityHuman, Mouse
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human Cytochrome P450 2B6 (401-491/491aa)
Antibody TypePrimary Antibody
Concentration1mg/ml
Dilution rangeWB=1:500-2000, Flow-Cyt=2ug/Test
Form/AppearanceLiquid
ConjugationUnconjugated
MW56 kDa
TargetCYP2B6
UniProt IDP20813
RRIDAB_10921819
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative names11B1; CP2B6_HUMAN; CPB 6; CPB6; CYP 2B6; CYP IIB6;
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NoteFor research use only
Expiration Date12 months from date of receipt.
Cytochrome P450 2B6 Rabbit Polyclonal Antibody

Blank control: U937. Primary Antibody (green line): Rabbit Anti-Cytochrome P450 2B6 antibody (orb5949), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Cytochrome P450 2B6 Rabbit Polyclonal Antibody

Sample: Liver (Mouse) Lysate at 40 ug, Primary: Anti-Cytochrome P450 2B6 (orb5949) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 56 kD, Observed band size: 56 kD.

Cytochrome P450 2B6 Rabbit Polyclonal Antibody

Sample: Lung (Mouse) Lysate at 40 ug, Primary: Anti-Cytochrome P450 2B6 (orb5949) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 56 kD, Observed band size: 56 kD.

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