Cytochrome P450 17A1 Rabbit Polyclonal Antibody

SKU: orb101132

Description

Cytochrome P450 17A1 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Mouse, Rat
• Predicted Reactivity: Bovine, Equine, Human, Rabbit, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human P45017A1/Cytochrome P450 17A1 (24-65/508aa)
• Target: CYP17A1
• Molecular Weight: 57 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

20 lyase; CP17A_HUMAN; CPT7; CYP17; CYP17A1; CYPXVII; Cytochrome P450 17A1; Cytochrome P450 family 17; Cytochrome P450 family 17 subfamily A polypeptide 1; Cytochrome p450 XVIIA1; Cytochrome p450, subfamily XVII(steroid 17 alpha hydroxylase) adrenal hyperplasia; Cytochrome P450-C17; Cytochrome P450c17; P450 C17; P450c17; S17AH; Steroid 17 alpha hydroxylase/17, 20 lyase; Steroid 17 alpha monooxygenase; Steroid 17-alpha-hydroxylase/17; Steroid 17-alpha-monooxygenase.

Paraformaldehyde-fixed, paraffin embedded (rat testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Cytochrome P450 17A1) Polyclonal Antibody, Unconjugated (orb101132) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Brain (Rat) Lysate at 40 ug, Primary: Anti-Cytochrome P450 17A1 (orb101132) at 1/300 dilution, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1/5000 dilution, Predicted band size: 57 kD, Observed band size: 57 kD.

Sample: Lane 1: Mouse Adrenal gland tissue lysates, Lane 2: Rat Adrenal gland tissue lysates, Primary: Anti-Cytochrome P450 17A1 (orb101132) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 57 kDa, Observed band size: 60 kDa.

Sample: Testis (Mouse) Lysate at 40 ug, Primary: Anti-Cytochrome P450 17A1 (orb101132) at 1/300 dilution, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1/5000 dilution, Predicted band size: 57 kD, Observed band size: 57 kD.

Quick Database Links

Gene Symbol

CYP17A1

UniProt

P05093