Cytochrome b245 Light Chain Rabbit Polyclonal Antibody

SKU: orb5035

Description

Cytochrome b245 Light Chain Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CK18 Cytochrome b245 Light Chain/p22phox (21-120/195aa)
• Target: CYBA
• Molecular Weight: 21 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

CYBA; Cytochrome b 245 alpha polypeptide; Cytochrome b 245 light chain; Cytochrome b alpha polypeptide; Cytochrome b(558) alpha chain; Cytochrome b(558) alpha subunit; Cytochrome b558 subunit alpha; Flavocytochrome b 558 alpha polypeptide; Neutrophil cytochrome b 22 kDa polypeptide; p22 phagocyte B-cytochrome; CY24A_HUMAN; p22-phox; p22phox; Superoxide-generating NADPH oxidase light chain subunit; p22-PHOX.

Tissue/Cell: human liver carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-CYBA Polyclonal Antibody, Unconjugated (orb5035) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

CYBA

UniProt

P13498