Cys-Protein-G Protein

SKU: orb425011

Description

Cys-Protein G is produced in E. coli as a single, non-glycosylated polypeptide consisting of 201 amino acids with an N-terminal cysteine residue. It has a predicted molecular weight of approximately 21.9 kDa, but migrates with an apparent molecular weight of about 40 kDa on SDS-PAGE.

Images & Validation

• Application Notes:
  Recombinant & Natural Proteins

Key Properties

• Source: Escherichia Coli
• Protein Sequence: CLPKTDTYKL ILNGKTLKGE TTTEAVDAAT AEKVFKQYAN DNGVDGEWTY DDATKTFTVT EKPEVIDASE LTPAVTTYKL VINGKTLKGE TTTEAVDAAT AEKVFKQYAN DNGVDGEWTY DDATKTFTVT EKPEVIDASE LTPAVTTYKL VINGKTLKGE TTTKAVDAET AEKAFKQYAN DNGVDGVWTY DDATKTFTVT E
• Purification: proprietary chromatographic techniques
• Purity: Greater than 95.0% as determined by SDS-PAGE and HPLC analyses.

Storage & Handling

• Storage: Stability: Lyophilized Protein G although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Protein G should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles
• Form/Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Buffer/Preservatives: Lyophilized without any additives.
• Reconstitution: It is recommended to reconstitute the lyophilized Protein G in sterile 18 MΩ·cm water at a concentration of no less than 100 µg/mL.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only