CYP7A1 Rabbit Polyclonal Antibody

• Catalog Number: orb5022
• Category: Antibodies
• Description: CYP7A1 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P
• Predicted Reactivity: Mouse, Porcine, Rabbit, Rat
• Reactivity: Guinea pig, Human
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CYP7A1 (351-400/504aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 55 kDa
• Target: CYP7A1
• UniProt ID: P22680
• RRID: AB_10927729
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Cholesterol 7 alpha hydroxylase; Cholesterol 7 alp
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with CYP7A1 Polyclonal Antibody, Unconjugated (orb5022) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Liver, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with CYP7A1 Polyclonal Antibody, Unconjugated (orb5022) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.