CYP46A1 Antibody

SKU: orb1255758

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Description

CYP46A1 Antibody

Images & Validation

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Item 1 of 2

Tested Applications	IF, IHC, WB
Reactivity	Human, Mouse, Rat
Application Notes	WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 24-280 of human CYP46A1 (NP_006659.1).
Target	CYP46A1
Molecular Weight	Observed: 57kDa
Purification	Affinity purification

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Liquid
Concentration	batch dependent
Disclaimer	For research use only

Alternative Names

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CYP46A1, CP46, CYP46

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Western blot analysis of extracts of various cell lines, using CYP46A1 antibody (orb1255758) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunohistochemistry of paraffin-embedded human prostate using CYP46A1 antibody (orb1255758) at dilution of 1:100 (40x lens).

Quick Database Links

Gene Symbol

CYP46A1

UniProt

Q9Y6A2

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunofluorescence

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