CYP2E1 Rabbit Polyclonal Antibody

• Catalog Number: orb100476
• Category: Antibodies
• Description: CYP2E1 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Equine, Porcine, Rabbit, Sheep
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CYP2E1 (401-493/493aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 57 kDa
• Target: CYP2E1
• UniProt ID: P05181
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: 4-nitrophenol 2-hydroxylase; CP2E1_HUMAN; CPE 1; C
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Iwao, Yasuhito et al. Liver atrophy after percutaneous transhepatic portal embolization occurs in two histological phases: Hepatocellular atrophy followed by apoptosis World J Hepatol, 9, 1227-1238 (2017)

PubMed: 29184609

Blank control: U937. Primary Antibody (green line): Rabbit Anti-CYP2E1 antibody (orb100476), Dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE, Dilution: 1 µg/test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Hela (Human) Cell Lysate at 30 ug, Primary: Anti-CYP2E1 (orb100476) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 57 kD, Observed band size: 57 kD.

Sample: HepG2 (Human) Cell Lysate at 30 ug, Primary: Anti-CYP2E1 (orb100476) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 57 kD, Observed band size: 57 kD.

Sample: Liver (Mouse) Lysate at 40 ug, Primary: Anti-CYP2E1 (orb100476) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 57 kD, Observed band size: 57 kD.

Tissue/cell: rat kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-CYP2E1 Polyclonal Antibody, Unconjugated (orb100476) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.