CYP2E1 Rabbit Polyclonal Antibody

SKU: orb100476

Description

CYP2E1 Rabbit Polyclonal Antibody

Research Area

Alcohol Metabolism, Cancer Research, Drug Metabolism, Metabolism

Images & Validation

• Tested Applications: FC, IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1:100-500
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Porcine, Rabbit, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CYP2E1 (401-493/493aa)
• Target: CYP2E1
• Molecular Weight: 57 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

4-nitrophenol 2-hydroxylase; CP2E1_HUMAN; CPE 1; CPE1; CYP2E; CYP2E1; CYP2E1; CYPIIE1; Cytochrome P450 2E1; cytochrome P450 family 2 subfamily E polypeptide 1; Cytochrome P450 isozyme 3A; Cytochrome P450 LM3A; Cytochrome P450 subfamily IIE(ethanol inducible) polypeptide 1; Cytochrome P450, family 2, subfamily e, polypeptide 1; cytochrome P450, subfamily IIE(ethanol-inducible), polypeptide 1; Cytochrome P450, subfamily IIE; Cytochrome P450-ALC; Cytochrome P450-J; Cytochrome P450RLM6; EC 1.14.13.-; EC 1.14.13.n7; Ethanol inducible p450; Ethanol inducible p450; Ethanol-inducible P450; flavoprotein-linked monooxygenase; MGC133529; microsomal monooxygenase; OTTHUMP00000020813; OTTHUMP00000046571; P450 ALC; P450 J; P450-J; P450C2E; P450C2E; P450J; P450RLM6; xenobiotic monooxygenase.

Blank control: U937. Primary Antibody (green line): Rabbit Anti-CYP2E1 antibody (orb100476), Dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE, Dilution: 1 µg/test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Hela (Human) Cell Lysate at 30 ug, Primary: Anti-CYP2E1 (orb100476) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 57 kD, Observed band size: 57 kD.

Sample: HepG2 (Human) Cell Lysate at 30 ug, Primary: Anti-CYP2E1 (orb100476) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 57 kD, Observed band size: 57 kD.

Sample: Liver (Mouse) Lysate at 40 ug, Primary: Anti-CYP2E1 (orb100476) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 57 kD, Observed band size: 57 kD.

Tissue/cell: rat kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-CYP2E1 Polyclonal Antibody, Unconjugated (orb100476) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

CYP2E1

UniProt

P05181

Iwao, Yasuhito et al. Liver atrophy after percutaneous transhepatic portal embolization occurs in two histological phases: Hepatocellular atrophy followed by apoptosis World J Hepatol, 9, 1227-1238 (2017)

PubMed: 29184609