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Catalog Number | orb308843 |
---|---|
Category | Antibodies |
Description | CYP27B1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human CYP27B1 (475-508aa HFEVQPEPGAAPVRPKTRTVLVPERSINLQFLDR), different from the related mouse and rat sequences by six amino acids. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, By HeatWestern blot, 0.1-0.5μg/ml, Human, Mouse, Rat |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 56504 MW |
UniProt ID | O15528 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | 25-hydroxyvitamin D-1 alpha hydroxylase, mitochond Read more... |
Note | For research use only |
Application notes | Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
WB analysis of CYP27B1 using anti-CYP27B1 antibody.Lane 1:human HeLa cell;2:human Caco-2 cell;3:rat heart tissue;4:rat brain tissue;5:mouse heart tissue;6:mouse RAW264.7 cell.
IHC analysis of CYP27B1 using anti-CYP27B1 antibody. CYP27B1 was detected in a paraffin-embedded section of rat kidney tissue.
IHC analysis of CYP27B1 using anti-CYP27B1 antibody. CYP27B1 was detected in a paraffin-embedded section of human kidney cancer tissue.
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