CYP27B1 Antibody

• Catalog Number: orb1258759
• Category: Antibodies
• Description: CYP27B1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 25-195 of human CYP27B1 (NP_000776.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IF: 1:20 - 1:50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 63kDa
• Target: CYP27B1
• UniProt ID: O15528
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: CP2B, CYP1, CYP1alpha, CYP27B, P450c1, PDDR, VDD1,
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IF: 1:20 - 1:50
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using CYP27B1 antibody (orb1258759) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunofluorescence analysis of HeLa cells using CYP27B1 antibody (orb1258759) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.