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Description
Research Area
Metabolism Research
Images & Validation
−| Tested Applications | ELISA, IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution Range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000 |
| Reactivity | Mouse |
| Predicted Reactivity | Bovine, Canine, Equine, Human, Porcine, Rabbit, Rat |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human CYP27A1 (321-420/531aa) |
| Target | CYP27A1 |
| Molecular Weight | 57 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−CP27; CTX; CYP27; 1300013A03Rik; P450C27; CP27A_HUMAN; CYP27A1; 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol 26-hydroxylase; Cytochrome P-450C27/25; Cytochrome P450 27; Sterol 27-hydroxylase; Vitamin D(3) 25-hydroxylase; 1.14.15.15; CP27A_MOUSE; CP27A_RAT; cytochrome P450 family 27 subfamily A member 1; cytochrome P450, subfamily XXVIIA (steroid 27-hydroxylase, cerebrotendinous xanthomatosis), polypeptide 1; cytochrome P450, family 27, subfamily A, polypeptide 1; cerebrotendinous xanthomatosis
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Quick Database Links
Gene Symbol
CYP27A1
UniProt
UniProt Details
− No UniProt data available
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)








