CYP21 antibody

• Catalog Number: orb13362
• Category: Tools
• Description: CYP21 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-Fr, IHC-P
• Predicted Reactivity: Bovine, Canine, Equine, Human, Mouse, Porcine
• Reactivity: Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CYP21 (101-200/494 aa)
• Concentration: 1mg/ml
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 54 kDa
• Target: CYP21A2
• UniProt ID: P08686
• Storage: Shipped at 4°C. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol.
• Alternative names: Cytochrome P450 21; cytochrome P450, family 21, su
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Jauregui, Estela J. et al. Leydig cell genes change their expression and association with polysomes in a stage-specific manner in the adult mouse testis Biol Reprod, 98, 722-738 (2018)

PubMed: 29408990

Paraffin-embedded rat testis tissue fixed with 4% paraformaldehyde. Antigen retrieval by boiling with citrate buffer. Blocking buffer is goat serum (37 degrees for 20 min.). CYP21 antibody at 1:200 dilution with overnight incubation at 4 degrees