CYP11A1 Rabbit Polyclonal Antibody

• Catalog Number: orb5936
• Category: Antibodies
• Description: CYP11A1 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CYP11A1/P450SCC (321-420/521aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-5000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 53 kDa
• Target: CYP11A1
• UniProt ID: P05108
• RRID: AB_10941394
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Cholesterol 20 22 desmolase; Cholesterol desmolase
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: Placenta (Mouse) Lysate at 30 ug, Primary: Anti-CYP11A1 (Ser576) (orb5936) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution, Predicted band size: 53/57 kD, Observed band size: 57 kD.

Paraformaldehyde-fixed, paraffin embedded (mouse adrenal gland), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CYP11A1) Polyclonal Antibody, Unconjugated (orb5936) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat adrenal gland tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CYP11A1) Polyclonal Antibody, Unconjugated (orb5936) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat adrenal gland), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CYP11A1) Polyclonal Antibody, Unconjugated (orb5936) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Brain (Rat) Lysate at 30 ug, Lane 2: Kidney (Rat) Lysate at 30 ug, Primary: Anti-CYP11A1/P450SCC (orb5936) at 1:200 dilution, Secondary: HRP conjugated Goat Anti-Rabbit IgG (orb572747) at 1:3000 dilution, Predicted band size: 53kD, Observed band size: 53kD.