Cynomolgus IL-4 Protein

• Catalog Number: orb1152433
• Category: Proteins
• Description: Interleukin-4, is a cytokine that induces differentiation of naive helper T cells (Th0 cells to Th2 cells). In the presence of IL-4 and IL-13, cytokines that are produced in a Th-2 type response, particularly during allergy and parasitic infections, macrophages become differentially activated, And this cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. Recently, researcher found that the cytokine IL-4 plays a key role in development of innate CD8+ T cells in the thymus of several gene-deficient mouse strains, including Itk, KLF2, CBP and Id3, without previous exposure to antigen.
• Reactivity: Monkey
• Tag: C-Fc
• Form/Appearance: Powder
• Purity: 90%
• Conjugation: Unconjugated
• MW: 41.4 kDa
• Target: IL-4
• Source: Cynomolgus IL-4, Fc Tag (orb1152433) is expressed from human 293 cells (HEK293). It contains AA His 25 - Ser 153 (Accession # P79339-1).
• Expression System: HEK293
• Biological Activity: Immobilized Cynomolgus / Rhesus macaque IL-4 R alpha, His Tag at 5 μg/mL (100 μL/well) can bind Cynomolgus IL-4, Fc Tag with a linear range of 0.1-4 ng/mL (QC tested).
• Expression Region: His 25 - Ser 153
• Endotoxins: 1.0 EU per μg
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: 51 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5
• Alternative names: IL4,BCGF1,BSF1
• Note: For research use only
• Application notes: This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 41.4 kDa. The protein migrates as 46-50 kDa and 53 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

Immobilized Cynomolgus / Rhesus macaque IL-4 R alpha, His Tag (Cat. No. orb257988) at 5 μg/mL (100 μL/well) can bind Cynomolgus IL-4, Fc Tag (Cat. No. orb1152433) with a linear range of 0.1-4 ng/mL (QC tested).