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Catalog Number | orb257323 |
---|---|
Category | Proteins |
Description | Cynomolgus CD3 epsilon, His Tag is expressed from human 293 cells (HEK293). It contains AA Gln 22 - Asp 117 (Accession # Q95LI5-1). |
Reactivity | Monkey |
Tag | C-10×His |
Form/Appearance | Powder |
Purity | 90% |
Conjugation | Unconjugated |
MW | 16.1 kDa |
Target | CD3 epsilon |
UniProt ID | Q95LI5 |
Protein Sequence | NP_001270544.1 |
Source | Cynomolgus CD3 epsilon, His Tag (orb257323) is expressed from human 293 cells (HEK293). It contains AA Gln 22 - Asp 117 (Accession # Q95LI5-1). |
Expression System | HEK293 |
Biological Origin | Cynomolgus |
Expression Region | Gln 22 - Asp 117 |
Endotoxins | 1.0 EU per μg |
NCBI | NP_001270544.1 |
Storage | -20°C |
Buffer/Preservatives | PBS, pH7.4 |
Alternative names | FLJ18683,T3E,TCRE,CD3E,CD3-epsilon Read more... |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 16.1 kDa. As a result of glycosylation, the protein migrates as 18-23 kDa under reducing (R) condition, and 33-40 kDa under non-reducing (NR) condition (SDS-PAGE). |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Monkey CD3 epsilon protein
Immobilized Anti Human CD3 MAb, mouse IgG1 at 2ug/mL (100 uL/well) can bind Cynomolgus CD3E, His Tag with a linear range of 0.4-6.25 ng/mL.
Unconjugated | |
95% | |
38.3 kDa | |
Cynomolgus CD3 epsilon, Fc,His Tag (orb257324) is expressed from human 293 cells (HEK293). It contains AA Gln 22 - Asp 117 (Accession # Q95LI5). |
98.00% | |
11.7 kDa (predicted). Due to glycosylation, the protein migrates to 13-16 kDa based on Tris-Bis PAGE result. |
98.00% | |
11.7 kDa (predicted). Due to glycosylation, the protein migrates to 14-16 kDa based on Tris-Bis PAGE result. |
> 95% by Tris-Bis PAGE | |
Recombinant Cynomolgus CD3 Epsilon Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Gln22-Asp117) of Cynomolgus CD3 Epsilon fused with a hFc His Tag tag at the C-terminal. |
> 95%by Bis-Tris PAGE, > 95%by HPLC | |
Recombinant Cynomolgus Cynomolgus CD3 Epsilon Protein is produced by mammalian expression system. The target protein is expressed with sequence (Gln22-Asp117) of cynomolgus Cynomolgus CD3 Epsilon (Accession #) fused with a His Tag at the C-terminus. |