CYK18 Antibody (C-term)

• Catalog Number: orb1787896
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Target: This CYK18 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 401-430 amino acids from the C-terminal region of human CYK18.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: 401-430 aa
• UniProt ID: P05783
• MW: 48058 Da
• Tested applications: FC, IF, IHC, WB
• Dilution range: IF: 1:25, IF: 1:25, IF: 1:25, WB: 1:2000, WB: 1:2000, WB: 1:2000, IHC: 1:25
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Keratin, type I cytoskeletal 18, Cell proliferatio
• Note: For research use only
• NCBI: NP_954657.1, NP_000215.1

All lanes: Anti-CYK18 Antibody (C-term) at 1:2000 dilution. Lane 1: Hela whole cell lysate. Lane 2: K562 whole cell lysate. Lane 3: A431 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 48 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-CYK18 Antibody (C-term) at 1:2000 dilution. Lane 1: Hela whole cell lysate. Lane 2: K562 whole cell lysate. Lane 3: A431 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 48 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-CYK18 Antibody (C-term) at 1:2000 dilution. Lane 1: Hela whole cell lysate. Lane 2: K562 whole cell lysate. Lane 3: A431 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 48 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling Pdx1 at 1/25 dilution, followed by Dylight 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoskeleton staining on HeLa cell line. The nuclear counter stain is DAPI (blue).

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling Pdx1 at 1/25 dilution, followed by Dylight 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoskeleton staining on HeLa cell line. The nuclear counter stain is DAPI (blue).

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling Pdx1 at 1/25 dilution, followed by Dylight 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoskeleton staining on HeLa cell line. The nuclear counter stain is DAPI (blue).

Staining CYK18 in human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.