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Cyclophilin F Rabbit Polyclonal Antibody

SKU: orb100803

Description

Cyclophilin F Rabbit Polyclonal Antibody

Research Area

Apoptosis, Cell Biology, Cell Cycle, Metabolism, Mitochondrial, Mitochondrial Markers

Images & Validation

Tested ApplicationsIF, IHC-Fr, IHC-P
Dilution RangeIHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
ReactivityHuman
Predicted ReactivityBovine, Gallus, Mouse, Porcine, Rat

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human Cyclophilin F (31-130/207aa)
TargetPPIF
Molecular Weight22 kDa
PurificationAffinity purified by Protein A
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Cyclophilin 3; cyclophilin D; Cyp D; CyP M; CyP-D; CyP-M; CYP3; CypD; hCyP3; mitochondrial; Mitochondrial cyclophilin; Peptidyl prolyl cis trans isomerase, mitochondral; Peptidyl-prolyl cis-trans isomerase F; Peptidyl-prolyl cis-trans isomerase F, mitochondrial; Peptidylprolyl isomerase F(cyclophilin F); Peptidylprolyl isomerase F; PPIase; PPIase F; Ppif; Rotamase; Rotamase F; PPIF_HUMAN.

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Cyclophilin F Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Cyclophilin F) Polyclonal Antibody, Unconjugated (orb100803) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

UniProt Details

No UniProt data available

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Protocol Information

IHC-P
Immunohistochemistry Paraffin
View Protocol
IHC-Fr
Immunohistochemistry Frozen
View Protocol
IF
Immunofluorescence
View Protocol

Cyclophilin F Rabbit Polyclonal Antibody (orb100803)

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Available Sizes

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50 μl
$ 250.00
100 μl
$ 390.00
200 μl
$ 470.00
DispatchUsually dispatched within 5-10 working days
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