Cyclooxygenase 2 Rabbit Polyclonal Antibody (FITC)

SKU: orb15422

Description

Cyclooxygenase 2 Rabbit Polyclonal Antibody (FITC)

Images & Validation

• Tested Applications: IF
• Dilution range: IF=1:100-500
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Porcine, Rabbit, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Cyclooxygenase 2 (501-604/604aa)
• Target: Ptgs2
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

PTGS2; COX 2; COX-2; Cyclooxygenase; Cyclooxygenase2; Cyclooxygenase-2; COX2; hCox 2; PGG/HS; PGH synthase 2; PGHS 2; PGHS2; PHS 2; PHS II; PHS2; Prostaglandin endoperoxide synthase 2; Prostaglandin G/H synthase 2 precursor; Prostaglandin G/H synthase and cyclooxygenase; Prostaglandin G/H synthase 2; Prostaglandin H2 synthase 2; TIS10II; PGH2_HUMAN.

Blank control (blue line): HepG2 (blue). Primary Antibody (green line): Rabbit Anti-Cyclooxygenase 2/FITC Conjugated antibody (orb15422), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG-FITC. Protocol, The cells were fixed with 2% paraformaldehyde (10 min), then permeabilized with 90% ice-cold methanol for 30 min on ice. The cells were then incubated in 1X PBS/2% BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

Quick Database Links

Gene Symbol

Ptgs2