Cyclin T1 Antibody

• Catalog Number: orb1524722
• Category: Antibodies
• Description: Cyclin T1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human
• Immunogen: between 425 and 475
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: Cyclin T1
• UniProt ID: O60563
• NCBI: NP_001231.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: CYCT1; cyclin-T1; HIVE1; MLLT10/CCNT1 fusion; cycl
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human Cyclin T1 by western blot. Samples: Whole cell lysate from HeLa (15 and 50 µg) and HEK293T (T; 50 µg) cells. Antibody: Affinity purified rabbit anti-Cyclin T1 antibody orb1524722 used for IP at 6 µg/mg lysate. Cyclin T1 was also used at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human Cyclin T1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Cyclin T1 antibody orb1524722 used for IP at 6 µg/mg lysate.

Detection of human Cyclin T1 by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Cyclin T1 (Cat. No. orb1524722) used at a dilution of 1: 200 (1µg/ml). Detection: DAB