Cyclin G Rabbit Polyclonal Antibody

• Catalog Number: orb10501
• Category: Antibodies
• Description: Cyclin G Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Porcine, Rabbit, Rat
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Cyclin G (181-295/295aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 34 kDa
• Target: CCNG1
• UniProt ID: P51959
• RRID: AB_10752982
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: CCNG 1; CCNG; CCNG1; CYCG1; Cyclin G; CCNG1_HUMAN;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

HepG2 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Cyclin G) polyclonal Antibody, Unconjugated (orb10501) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Sample: Kidney (Mouse) Lysate at 40 ug, Primary: Anti-Cyclin G (orb10501) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 34 kD, Observed band size: 34/30 kD.

Sample: Liver (Mouse) Lysate at 40 ug, Primary: Anti-Cyclin G (orb10501) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 34 kD, Observed band size: 34/30 kD.

Sample: Spleen (Mouse) Lysate at 40 ug, Primary: Anti-Cyclin G (orb10501) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 34 kD, Observed band size: 34/30 kD.