Cyclin D2 Rabbit Polyclonal Antibody

SKU: orb10497

Description

Cyclin D2 Rabbit Polyclonal Antibody

Research Area

Cell Biology, Cell Cycle, Cyclin D family, Cyclins

Images & Validation

• Tested Applications: FC, IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=5μg/Test
• Reactivity: Human, Rat
• Predicted Reactivity: Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from the middle of human Cyclin D2 (161-288/288aa)
• Target: CCND2
• Molecular Weight: 35 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

CCND 2; CCND2; CyclinD2; G1/S specific cyclin D2; KIAK0002; MGC102758; CCND2_HUMAN; G1/S-specific cyclin-D2.

The figure annotation: The blue histogram is unstained cells. The Wathet Blue histogram is cells stained with secondary antibody (orb868805) alone. The Orange histogram is cells stained with rabbit IgG isotype control (orb570250) antibody plus secondary antibody. The green histogram is cells stained with Rabbit Anti-Cyclin D2 antibody (orb10497) plus secondary antibody. Concebtration: 5 µg/10^6 cells. Positive control: MCF-7 cells.

Tissue/Cell: rat mammary tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Cyclin D2 Polyclonal Antibody, Unconjugated (orb10497) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, PE conjugated used at 1:200 dilution for 40 minutes at 37°C. DAPI (5 ug/ml, blue) was used to stain the cell nuclei.

Quick Database Links

Gene Symbol

CCND2

UniProt

P30279