Cyclin D2 Rabbit Polyclonal Antibody

• Catalog Number: orb10497
• Category: Antibodies
• Description: Cyclin D2 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-Fr, IHC-P
• Predicted Reactivity: Mouse
• Reactivity: Human, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from the middle of human Cyclin D2 (161-288/288aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=5μg/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 35 kDa
• Target: CCND2
• UniProt ID: P30279
• RRID: AB_10753061
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: CCND 2; CCND2; CyclinD2; G1/S specific cyclin D2;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

The figure annotation: The blue histogram is unstained cells. The Wathet Blue histogram is cells stained with secondary antibody (orb868805) alone. The Orange histogram is cells stained with rabbit IgG isotype control (orb570250) antibody plus secondary antibody. The green histogram is cells stained with Rabbit Anti-Cyclin D2 antibody (orb10497) plus secondary antibody. Concebtration: 5 µg/10^6 cells. Positive control: MCF-7 cells.

Tissue/Cell: rat mammary tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Cyclin D2 Polyclonal Antibody, Unconjugated (orb10497) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, PE conjugated used at 1:200 dilution for 40 minutes at 37°C. DAPI (5 ug/ml, blue) was used to stain the cell nuclei.