CYC1 Antibody

• Catalog Number: orb1265032
• Category: Antibodies
• Description: CYC1 Antibody
• Target: CYC1
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Immunogen: This CYC1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 142-176 amino acids from the Central region of human CYC1.
• UniProt ID: P08574
• MW: 35 kDa
• Tested applications: IF, IHC-P, WB
• Application notes: For IF starting dilution is: 1:25For IHC-P starting dilution is: 1:25For WB starting dilution is: 1:1000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Cytochrome c1, heme protein, mitochondrial, Comple
• Note: For research use only
• NCBI: P08574

Fluorescent image of Hela cells stained with CYC1 Antibody. Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).

Immunohistochemical analysis of paraffin-embedded H. stomach section using CYC1 Antibody. Antibody was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded M. stomach section using CYC1 Antibody. Antibody was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded R. stomach section using CYC1 Antibody. Antibody was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Western blot analysis of lysates from A431, Hela, HepG2, MCF-7, U-251 MG cell lines and mouse brain tissue lysate (from left to right), using CYC1 Antibody at 1:1000 at each lane.