Cybr Antibody

• Catalog Number: orb750516
• Category: Antibodies
• Description: Cytip antibody
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Antiserum
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Lyophilized
• Concentration: 85 mg/mL
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Purity: This Cybr antibody is directed against mouse Cybr. The product is delipidated and defibrinated antiserum. A BLAST analysis was used to suggest cross-reactivity with Cybr from mouse and rat based on a 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
• Immunogen: PSCDBP antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region in the C-terminal portion of mouse Cybr.
• UniProt ID: Q91VY6
• Tested applications: ELISA, IP, WB
• Dilution range: ELISA: 1:5,000 - 1:20,000, IP: 1:100, WB: 1:1,000 - 1:10,000
• Application notes: This antibody has been tested for use in ELISA and immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 40 kDa in size corresponding to Cybr by western blotting after IP in the appropriate cell lysate or extract. To date, this antibody has not shown direct detection of endogenous or over-expressed Cybr protein, but is capable of immunoprecipitating the antigen from lysates. It is possible, but has not yet been confirmed, that this antibody recognizes a conformational epitope.
• Antibody Type: Primary Antibody
• Storage: Store PSCDBP antibody / Anti-Cybr at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Alternative names: rabbit anti-Cybr antibody, Cytohesin binder and re
• Note: For research use only
• NCBI: 21105855

Western blot using Biorbyt's anti-Cybr antibody shows detection of recombinant Cybr using anti-HA tag antibody after immunoprecipitation using anti-Cybr. Lane 5 contains lysate from control HEK293 cells. Lane 6 shows IP/WB of HEK293 cells expressing Cybr-HA. Lane 7 and 8 are loaded similarly to lanes 5 and 6 but were immunoprecipitated with anti-HA. Lane 9 contains marker. Lane 10 contains purified CybrHA.