Cybr Antibody

SKU: orb345590

Description

Cybr antibody

Images & Validation

• Tested Applications: ELISA, WB
• Dilution range: ELISA: 1:5,000 - 1:20,000, WB: 1:1000
• Reactivity: Mouse
• Application Notes:
  This antibody has been tested for use in ELISA, western blot, and immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 40 kDa in size corresponding to Cybr by western blotting in the appropriate cell lysate or extract. This antibody detects endogenous and over-expressed Cybr protein and is capable of immunoprecipitating the antigen from lysates.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region in the carboxy terminal portion of mouse Cybr.
• Purity: This affinity purified antibody is directed against mouse Cybr. A BLAST analysis was used to suggest cross-reactivity with Cybr from mouse and rat based on a 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 0.86 mg/mL
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-Cybr Antibody, Cytohesin-interacting protein, Cytohesin-binding protein HE, Cbp HE, Pleckstrin homology Sec7 and coiled-coil domains-binding protein, Pscdbp, Cytip

Western blot using Biorbyt's affinity purified anti-Cybr antibody shows detection of endogenous Cybr from mouse splenocytes using anti-Cybr antibody to immunoprecipitate and western blot (lanes 1-3). Lane 1 shows reactivity of pre-immune sera. Lane 2 shows endogenous Cybr detected with antibody. Lane 3 shows no band detected in lysates prepared from splenocytes of Cybr knock-out mouse. Lane 5 shows direct western blot of wt splenocytes. Lane 6 shows direct western blot of knock out mouse.

Quick Database Links

UniProt

Q91VY6

RefSeq:21105855