CYBR Antibody

• Catalog Number: orb1247637
• Category: Antibodies
• Description: CYBR Antibody
• Target: CYTIP
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Canine, Porcine
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: SLQRLLQHSSNGN-C
• UniProt ID: O60759
• Tested applications: ELISA, IF, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 38kDa band observed in lysates of cell line Jurkat and approx 40kDa in ysates of cell line MOLT4 (calculated MW of 40kDa according to NP_004279.3). Recommended concentration: 0.3-1ug/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Expression of the protein seen in the cytoplasm and nucleus of A431 and U251 cells. Recommended concentration: 10ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CYTIP, PSCDBP, CYBR, pleckstrin homology, Sec7 and
• Note: For research use only
• NCBI: NP_004279.3

orb1247637 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic and nuclear staining.

orb1247637 Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing strong cytoplasmic and weak nuclear staining.

orb1247637 (0.5 ug/ml) staining of Jurkat (A) and (1 ug/ml) MOLT4 (B) cell lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.