CYB5R4 Rabbit Polyclonal Antibody

• Catalog Number: orb182826
• Category: Antibodies
• Description: CYB5R4 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Porcine, Sheep
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CYB5R4 (21-120/521aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 59 kDa
• Target: CYB5R4
• UniProt ID: Q7L1T6
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: b5+b5R; b5b5r; C79736; cb5/cb5R; Cb5cb5r; Cyb5r4;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: Testis (Mouse) Lysate at 40 ug, Testis (Rat) Lysate at 40 ug, Primary: Anti-CYB5R4 (orb182826) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 59 kD, Observed band size: 59 kD.

Tissue/Cell: human laryngocarcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-CYB5R4 Polyclonal Antibody, Unconjugated (orb182826) 1:400, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.