CXorf36 Rabbit Polyclonal Antibody

SKU: orb10491

Description

CXorf36 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Mouse
• Predicted Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CXorf36 (101-182/182aa)
• Target: DIPK2B
• Molecular Weight: 45 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Deleted in autism 1 related protein; DIA1R; EPQL1862; hCG_1981635; hCG1981635; PRO3743; UPF0672 protein CXorf36; chromosome X open reading frame 36, isoform CRA_a; uncharacterized protein Cxorf36 precursor; PRO3743; EPQL1862; FLJ14103; FLJ55198; FLJ55198, ; bA435K1.1; 4930578C19Rik; DKFZp313K0825; DIA1R_HUMAN; CXorf36.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CXorf36) Polyclonal Antibody, Unconjugated (orb10491) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Kidney (Mouse) lysate at 60 ug, Liver (Mouse) lysate at 60 ug, Primary: Anti-CXorf36 (orb10491) at 1:300, Secondary: HRP conjugated Goat-Anti-Rabbit IgG (orb572747) at 1:5000, Predicted band size: 45 kD, Observed band size: 50/75 kD.

Sample: Lane 1: Mouse Cerebellum tissue lysates, Lane 2: Mouse Cerebrum tissue lysates, Primary: Anti-CXorf36 (orb10491) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 45 kDa, Observed band size: 46 kDa.

Quick Database Links

Gene Symbol

DIPK2B

UniProt

Q9H7Y0