CXCR2 Antibody

• Catalog Number: orb1671589
• Category: Antibodies
• Description: CXCR2 Antibody
• Clonality: Recombinant
• Clone Number: HC2
• Tested applications: FC
• Reactivity: Human
• Isotype: IgG kappa
• Immunogen: This antibody was raised by immunizing mice with human IL-8 (interleukin-8) receptor, type B.
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: CXCR2
• UniProt ID: P25025
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: CXCR-2, CD182, C-X-C chemokine receptor type 2, CX
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry using the Anti-IL-8RB antibody HC2. Paraformaldehyde fixed human peripheral blood monocytes were stained with anti-unknown specificity antibody (3.0; isotype control - black line) or the r version of HC2 (orb1671589 - blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of fixed human peripheral blood leukocytes with anti-IL-8RB antibody HC2. Immunofluorescence analysis of paraformaldehyde fixed human peripheral blood leukocytes on Shi-fix™ coverslips stained with the chimeric r version of HC2 (orb1671589) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671589- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (.1) followed by staining with Alexa Fluor® 488 secondary antibody.