CXCL9 Rabbit Polyclonal Antibody

• Catalog Number: orb783380
• Category: Antibodies
• Description: CXCL9 Rabbit Polyclonal Antibody
• Target: CXCL9
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Mouse
• Predicted Reactivity: Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: KLH conjugated synthetic peptide derived from human CXCL9 (23-125/125aa)
• UniProt ID: Q07325
• MW: 14 kDa
• Tested applications: ELISA, WB
• Dilution range: WB=1:500-2000, ELISA=1:5000-10000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: MIG; CMK; C-X-C motif chemokine 9; CXCL9; chemokin
• Note: For research use only

Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CXCL9) Polyclonal Antibody, Unconjugated (orb783380) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Recombinant mouse CXCL9 protein, Primary: Anti-CXCL9 (orb783380) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 14 kDa, Observed band size: 20 kDa.

Tissue/Cell: human spleen tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-CXCL9/MIG Polyclonal Antibody, Unconjugated (orb783380) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.