CXCL13/BCA1 Rabbit Polyclonal Antibody

SKU: orb101825

Description

CXCL13/BCA1 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: FC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test
• Reactivity: Human
• Predicted Reactivity: Canine

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CXCL13/BCA1 (23-109/109aa)
• Target: CXCL13
• Molecular Weight: 10 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

C-X-C motif chemokine 13; Angie; ANGIE; ANGIE2; B cell-attracting chemokine 1; B cell attracting chemokine 1; B lymphocyte chemoattractant; B-cell chemoattractant; B-cell-attracting chemokine 1; B-cell-homing chemokine(ligand for Burkitt's lymphoma receptor-1); CXL13_MOUSE; BCA-1; BLC; BLR1L; Chemokine(C-X-C motif) ligand 13; Chemokine(C-X-C motif) ligand 13(B-cell chemoattractant); Chemokine, CXC motif, ligand 13; CXC chemokine BLC; CXCL13; SCYB13; Small inducible cytokine B subfamily(Cys-X-Cys motif), member 13(B-cell chemoattractant); Small-inducible cytokine B13; Small inducible cytokine B13; Small inducible cytokine subfamily B, member 13.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CXCL13) Polyclonal Antibody, Unconjugated (orb101825) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Human Jurkat cell lysates, Primary: Anti-CXCL13 (orb101825) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 10 kDa, Observed band size: 16 kDa.

Quick Database Links

Gene Symbol

CXCL13

UniProt

O43927

References

Worthmann, K. et al. Pathogenetic role of glomerular CXCL13 expression in lupus nephritis Clin Exp Immunol, 178, 20-27 (2014)

PubMed: 24827905