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CXCL13/BCA1 Rabbit Polyclonal Antibody

Catalog Number: orb101825

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb101825
CategoryAntibodies
DescriptionCXCL13/BCA1 Rabbit Polyclonal Antibody
TargetCXCL13
ClonalityPolyclonal
Species/HostRabbit
ConjugationUnconjugated
ReactivityHuman
Predicted ReactivityCanine
Form/AppearanceLiquid
Concentration1mg/ml
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
ImmunogenKLH conjugated synthetic peptide derived from mouse CXCL13/BCA1 (23-109/109aa)
UniProt IDO55038
MW10 kDa
Tested applicationsFC, IF, IHC-Fr, IHC-P, WB
Dilution rangeWB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test
Antibody TypePrimary Antibody
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesC-X-C motif chemokine 13; Angie; ANGIE; ANGIE2; B
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NoteFor research use only

Worthmann, K. et al. Pathogenetic role of glomerular CXCL13 expression in lupus nephritis Clin Exp Immunol, 178, 20-27 (2014)

CXCL13/BCA1 Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CXCL13) Polyclonal Antibody, Unconjugated (orb101825) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

CXCL13/BCA1 Rabbit Polyclonal Antibody

Sample: Lane 1: Human Jurkat cell lysates, Primary: Anti-CXCL13 (orb101825) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 10 kDa, Observed band size: 16 kDa.

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    FC,  IF

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    FC,  IF

    Canine

    Human

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    100 μl