CXCL13/BCA1 Rabbit Polyclonal Antibody

• Catalog Number: orb101825
• Category: Antibodies
• Description: CXCL13/BCA1 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Canine
• Reactivity: Human
• Immunogen: KLH conjugated synthetic peptide derived from mouse CXCL13/BCA1 (23-109/109aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 10 kDa
• Target: CXCL13
• UniProt ID: O55038
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: C-X-C motif chemokine 13; Angie; ANGIE; ANGIE2; B
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Worthmann, K. et al. Pathogenetic role of glomerular CXCL13 expression in lupus nephritis Clin Exp Immunol, 178, 20-27 (2014)

PubMed: 24827905

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CXCL13) Polyclonal Antibody, Unconjugated (orb101825) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Human Jurkat cell lysates, Primary: Anti-CXCL13 (orb101825) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 10 kDa, Observed band size: 16 kDa.