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CXCL13 Rabbit Polyclonal Antibody

Catalog Number: orb13426

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb13426
CategoryAntibodies
DescriptionCXCL13 Rabbit Polyclonal Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsFC, IF, IHC-Fr, IHC-P, WB
Predicted ReactivityCanine
ReactivityHuman
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human CXCL13 (23-109/109aa)
Antibody TypePrimary Antibody
Concentration1mg/ml
Dilution rangeWB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test
Form/AppearanceLiquid
ConjugationUnconjugated
MW10 kDa
TargetCXCL13
UniProt IDO43927
RRIDAB_10753200
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesANGIE; ANGIE2; B cell attracting chemokine 1; B ce
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NoteFor research use only
Expiration Date12 months from date of receipt.
CXCL13 Rabbit Polyclonal Antibody

Blank control (black line): HepG2. Primary Antibody (green line): Rabbit Anti-CXCL13 antibody (orb13426), Dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488, Dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

CXCL13 Rabbit Polyclonal Antibody

Human breast tumor tissue stained with Rabbit Anti-CXCL13 Polyclonal Antibody (orb13426).

CXCL13 Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CXCL13) Polyclonal Antibody, Unconjugated (orb13426) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

CXCL13 Rabbit Polyclonal Antibody

Sample: Lane 1: Human Jurkat cell lysates, Primary: Anti-CXCL13 (orb13426) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 10 kDa, Observed band size: 16 kDa.

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