CUL5 antibody

• Catalog Number: orb378019
• Category: Antibodies
• Description: Rabbit polyclonal antibody to CUL5.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant full length protein of human Cullin 5
• Dilution range: WB: 1:500-2000, IF/ICC: 1:50-200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: CUL5
• Entrez: 75717, 64624, 8065
• UniProt ID: Q9JJ31, Q9D5V5, Q93034
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: VACM1; Cullin-5; CUL-5; Vasopressin-activated calc
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Cullin 5 expression in K562 (A), PC3 (B), SW480 (C), mouse brain (D), mouse heart (E) whole cell lysates. (Predicted band size: 90 kD; Observed band size: 100 kD)

Immunofluorescent analysis of Cullin 5 staining in SW480 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.