Cul5 Antibody

• Catalog Number: orb1527084
• Category: Antibodies
• Description: Cul5 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: between 1 and 50
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 5 - 15 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: Cul5
• UniProt ID: Q93034
• NCBI: NP_003469.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: VACM-1; CUL-5; Vasopressin-activated calcium-mobil
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human Cul5 by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, LNCaP, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Cul5 antibody orb1527084 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of mouse Cul5 by western blot. Samples: Whole cell lysate (10 µg) from NIH 3T3, CT26, and CH27 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Cul5 antibody orb1527084 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human Cul5 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Cul5 antibody orb1527084 used for IP at 6 µg per reaction.

Detection of human Cul5 by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Cul5 (Cat. No. orb1527084) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB